Features of Benchtop Bioreactors

Features	Description and function
Main switch	Located on the right-hand side of the basic unit. It lights up green as soon as it is switched on.
LED strip	Located on the front of the basic unit Lights up green as soon as the equipment is switched on. The equipment is functioning as normal. Flashes green. This indicates a parameter alarm operation section Flashes red. This indicates an Equipment error interferences
Pumps	Pump drive located on the left-hand side of the basic unit. Supplied reagents and feed solution via four peristaltic pumps Pump 1: Acid (digital) Alternative setting: FEED (analogue) Pump 2: Base (digital) Alternative setting: FEED (analogue) Pump 3: AF (antifoam, digital) Alternative setting: LEVEL (digital) or FEED (analogue) Pump 4: Feed (analogue) Alternative setting: BALANCED (analogue)
Identification plate	Located at the side of the basic equipment. Allow clear identification of the equipment
Power connection	Located on the rear of the basic unit, at the bottom left. Connection socket for the power cable
Water connections	Located on the rear of the basic unit, at the bottom right. They are marked with the following symbols: H2O IN: water inlet H2O OUT: water outlet
Gas connections	Located on the rear of the basic unit, at the bottom right, above the water connections. They are marked with the following symbols: Inlet oxygen / nitrogen Inlet air
Signal connections	Located on the rear of the basic unit, at the bottom left: The following signal connections with the corresponding symbols: SERVICE: 9-pin RS232, for connecting a diagnostic computer for maintenance. BALANCE: 9-pin RS232, for connecting a balance LAN port: for connecting a network cable
Motor cable connections	Located on the rear of the basic unit at the top left, and marked with a corresponding symbol.
Connections for sensors	The basic unit is equipped as standard for measuring temperature, pH, pO2 and antifoam. 1. pO2 2. Temperature (Pt100) 3. Antifoam 4. pH 5. Spare connection for turbidity measurement sensor (option)
Sparger connection (Gassing)	The connection for the gassing (sparger) is located on the front of the basic unit, at the bottom left. It is marked with a corresponding symbol.
Connections and water flow control valve for the exit gas cooler	The water connections for the exit gas cooler are located on the front of the basic unit, at the bottom left. They are marked with the corresponding symbols: 1. Exit gas cooler water outlet 2. Exit gas cooler water inlet The water flow control valve is located on the rear of the basic unit and marked with a corresponding symbol: The control valve is set at the factory. If necessary, the water flow rate can be set manually using the control valve. Turn anti-clockwise to increase the water flow rate Turn clockwise to reduce the flow rate The valve can be fixed in the required position using a lock nut.
Operating panel	The operating panel on the top right of the basic unit has a 7” TFT color touch screen. On the right-hand side of the panel is a USB port. On the left-hand side is a slot for an SD card The operating panel is switched on using the main switch.
Culture vessel	The culture vessel comprises a glass vessel, the top plate with the standard fittings (which vary based on vessel size) and the vessel holder with handles. The vessel is made of borosilicate glass.
Temperature control system	The temperature (heating and cooling) is controlled using a thermal block and its adapter. 1 Thermal block adapter 2 Thermal block 3 Fastening screw (Allen screw, 4 pieces) 4 Hook, 2 pieces
Stirrer	The stirrer shaft is driven from above and turns anti-clockwise (left when viewing the vessel from above). 1. Drive hub 2. Stirrer shaft 3. Mechanical seal
Gassing system	Gas Entry A silicone hose leads the gas or gas mixture from the gassing connection on the basic unit to the culture vessel, via a sterile filter. The gas is fed directly into the medium via the sparger (sparger gassing). Exit Gas Even without active gassing, any cultivation can increase the pressure inside the vessel through heating or gas production. As such, an exit gas line is essential for all cultivation processes.
pH control	pH in the medium is measured by the pH sensor and regulated by addition of reagents (acid, base).
pO2 control	The oxygen saturation of the (culture) medium is measured by the pO2 sensor and can be adjusted.
Antifoam control	The antifoam agent is kept in a reagent bottle that is connected to the antifoam sensor and the antifoam pump via a hose. The sensor also acts as a dosing needle. When the sensor comes in contact with foam, the antifoam pump is activated, and antifoam agent is fed into the vessel via the dosing needle.