Operation Principle of Bioreactor

     

Modes of operation	Descriptions
Batch	At the start of the fermentation run, each component is added to a fixed bioreactor capacity. To avoid nutrient constraint, which could reduce final biomass and product concentration, high substrate concentrations must be provided at first. Conditions change throughout the run as substrate consumption rises as cell concentration rises. With the exception of supplying gas for aeration and base to adjust pH, there is no addition of substrate or medium during the course of the bioprocess. The entire batch is taken for downstream processing at the conclusion of the run, and the reactors are then set up for the new fermentation process.
Fed-Batch	Mode of operation is a modified version of a batch fermentation process. It start after batch phase, where substrate decreases and cells grow exponentially  Before the substrate becomes limiting, feed (concentrated amino acids and glucose) is added at a rate of consumption throughout the remaining fermentation duration By controlling the rate of feed addition, high cell densities can be reached. Conditions in the bioreactor are kept constant, with the exception of the growing biomass, product and waste product concentrations Cells are growing and producing until the maximum density of the reactor is reached. By using low substrate concentrations in fed-batch mode, waste metabolism (lactate production) in the culture can be minimized or avoided.
Continuous	An open process that operates in the continuous mode has a steady inflow and outflow. Old culture medium and the cells are continuously removed, while new medium is continuously given. The amount of fresh medium or feed solution that can be fed to the culture during a process run has no bearing on the vessel's maximum operating capacity.

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