Modes of operation Descriptions Batch At the start of the fermentation run, each component is added to a fixed bioreactor capacity. To avoid nutrient constraint, which could reduce final biomass and product concentration, high substrate concentrations must be provided at first. Conditions change throughout the run as substrate consumption rises as cell concentration rises. With the exception of supplying gas for aeration and base to adjust pH, there is no addition of substrate or medium during the course of the bioprocess. The entire batch is taken for downstream processing at the conclusion of the run, and the reactors are then set up for the new fermentation process. Fed-Batch Mode of operation is a modified version of a batch fermentation process. It start after batch phase, where substrate decreases and cells grow exponentially Before the substrate becomes limiting, feed (concentrated amino acids and glucose) is added at a rate of consumption throughout the remaining ferme
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